Protein_Domain

Part:BBa_K2355025:Design

Designed by: Chrysillis Polhaus   Group: iGEM17_DTU-Denmark   (2017-10-26)


Cleavable peptide sequence from Human STE20/SPS1 related proline-alanine-rich protein kinase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This linker consists of just the identified cleavable oligopeptide, and there has thus not been made any modifications to optimise the function of this linker in regards to flexibility, length etc.


Source

The sequence comes from a Human STE20/SPS1 related proline-alanine-rich protein kinase (Uniprot accession number Q9UEW8) It is included in the JPT substrate screening set (JPT Peptide Technologies GmbH, Germany)

References